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mini trans blot turbotm semi dry transfer system  (Bio-Rad)


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    Structured Review

    Bio-Rad mini trans blot turbotm semi dry transfer system
    Mini Trans Blot Turbotm Semi Dry Transfer System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semi+dry+trans+blot+turbotm+transfer+system/Trans-Blot+Turbo+Transfer+System/bio_rxiv__64898__2026__05__04__722693-286-11-17
    Average 99 stars, based on 13558 article reviews
    mini trans blot turbotm semi dry transfer system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: SQSTM1/p62 accumulation is a hallmark of FLCN loss in Birt-Hogg-Dubé syndrome-associated kidney cancer
    Article Snippet: The samples were loaded on a 4–20% CriterionTM TGXTM Precast Gel (Bio-Rad, 5671093/5671094/5671095) and separation of protein by SDS gel-electrophoresis with 80V-150V. .. The proteins were transferred to a 0.45 μm LF PVDF membrane (Bio-Rad, 161–0374) using semi-dry Trans-Blot® TurboTM Transfer System (Bio-Rad) for 7 minutes at 25V. ..

    Article Title: Macrophages lacking TSC2 have mTORC1-dependent increased GPNMB and ameliorate ventricular dysfunction/remodeling after ischemia-reperfusion
    Article Snippet: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used for protein separation using 4–15% Criterion TGX Gel 18 W 30ul (Bio-Rad Cat# 5671084) or 4–20% Criterion TGX Gel 26 W 15ul (Bio-Rad Cat# 5671095). .. Proteins were blotted onto nitrocellulose membrane 0.45 μm, 8.5 × 13.5 cm (Bio-Rad Cat1620167) using a semi-dry Trans-Blot ® TurboTM Transfer System (BioRad Cat#1704150). .. Antibodies from Cell Signaling were used for P-P70S6 Kinase (9205, 1/000), P70S6 Kinase (9202, 1/1000), TSC2 (4308, 1/1000), P-4EBP1 (9451, 1/1000), 4EBP1 (9644, 1/1000), P-AKT (Ser473) (4060, 1/1000), P-AKT (Thr308) (5106, 1/1000), AKT (9272, 1/1000), and GPNMB (90205, 1/200).

    Article Title: Improved detection of lipidated Atg8a by immunoblotting in Drosophila melanogaster cells and tissues enables precise investigation of Atg8a flux and its termination.
    Article Snippet: The samples were separated by SDS-PAGE on 4–20% gradient gels (Bio-Rad, 567–1094, 567–1095). .. Proteins were then transferred onto LF PVDF membrane (Bio-Rad, 161–0374) using the semi-dry Trans-Blot® TurboTM Transfer System (Bio-Rad 1,704,150). ..

    Article Title: Macrophages lacking TSC2 have mTORC1-dependent increased GPNMB and ameliorate ventricular dysfunction/remodeling after ischemia-reperfusion.
    Article Snippet: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used for protein separation using 4–15% Criterion TGX Gel 18 W 30ul (Bio-Rad Cat# 5671084) or 4–20% Criterion TGX Gel 26 W 15ul (Bio-Rad Cat# 5671095). .. Proteins were blotted onto nitrocellulose membrane 0.45 μm, 8.5 × 13.5 cm (Bio-Rad Cat1620167) using a semi-dry Trans-Blot® TurboTM Transfer System (BioRad Cat#1704150). .. Antibodies from Cell Signaling were used for P-P70S6 Kinase (9205, 1/000), P70S6 Kinase (9202, 1/1000), TSC2 (4308, 1/1000), P-4EBP1 (9451, 1/1000), 4EBP1 (9644, 1/1000), P-AKT (Ser473) (4060, 1/1000), P-AKT (Thr308) (5106, 1/1000), AKT (9272, 1/1000), and GPNMB (90205, 1/200).

    Semi-Dry Trans-Blot:

    Article Title: SQSTM1/p62 accumulation is a hallmark of FLCN loss in Birt-Hogg-Dubé syndrome-associated kidney cancer
    Article Snippet: The samples were loaded on a 4–20% CriterionTM TGXTM Precast Gel (Bio-Rad, 5671093/5671094/5671095) and separation of protein by SDS gel-electrophoresis with 80V-150V. .. The proteins were transferred to a 0.45 μm LF PVDF membrane (Bio-Rad, 161–0374) using semi-dry Trans-Blot® TurboTM Transfer System (Bio-Rad) for 7 minutes at 25V. ..

    Article Title: Macrophages lacking TSC2 have mTORC1-dependent increased GPNMB and ameliorate ventricular dysfunction/remodeling after ischemia-reperfusion
    Article Snippet: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used for protein separation using 4–15% Criterion TGX Gel 18 W 30ul (Bio-Rad Cat# 5671084) or 4–20% Criterion TGX Gel 26 W 15ul (Bio-Rad Cat# 5671095). .. Proteins were blotted onto nitrocellulose membrane 0.45 μm, 8.5 × 13.5 cm (Bio-Rad Cat1620167) using a semi-dry Trans-Blot ® TurboTM Transfer System (BioRad Cat#1704150). .. Antibodies from Cell Signaling were used for P-P70S6 Kinase (9205, 1/000), P70S6 Kinase (9202, 1/1000), TSC2 (4308, 1/1000), P-4EBP1 (9451, 1/1000), 4EBP1 (9644, 1/1000), P-AKT (Ser473) (4060, 1/1000), P-AKT (Thr308) (5106, 1/1000), AKT (9272, 1/1000), and GPNMB (90205, 1/200).

    Article Title: Ion-Channel-Targeting Drugs for Chikungunya Virus.
    Article Snippet: .. The lysate was then resolved using electrophoresis, followed by the transfer of the antigens to nitrocellulose membranes using a Semi-dry Trans-Blot® TurboTM Transfer System (BIO-RAD; Hercules, CA, USA). .. The membranes were blocked with TBS-T (tris-buffered saline containing Tween-20) containing 1% low-fat milk, incubated overnight with a primary antibody (Anti-MBP, New England BioLabs), washed three times with TBS-T, incubated for two hours with a secondary antibody linked to horseradish peroxidase (anti-rabbit, Novagen; Temecula, CA, USA), and washed once again with TBS-T. Immunoreactive bands were detected using an ECL kit (Biological Industries; Haemek, Israel).

    Article Title: Improved detection of lipidated Atg8a by immunoblotting in Drosophila melanogaster cells and tissues enables precise investigation of Atg8a flux and its termination.
    Article Snippet: The samples were separated by SDS-PAGE on 4–20% gradient gels (Bio-Rad, 567–1094, 567–1095). .. Proteins were then transferred onto LF PVDF membrane (Bio-Rad, 161–0374) using the semi-dry Trans-Blot® TurboTM Transfer System (Bio-Rad 1,704,150). ..

    Article Title: Biological Evaluation of a Novel Compound with Predicted EZH2 and EED Binding Against Human Malignant Melanoma Cells
    Article Snippet: Next, whole cell extracts (60 μg) were prepared and subjected to electrophoresis on FastGene® PAGE precast gels (gradient: 4–20%), using a FastGene® PAGE Protein system (Nippon Genetics, Düren, Germany). .. Separated proteins were transferred to PVDF membranes using the semi-dry Trans-Blot® TurboTM transfer system (Bio-Rad Laboratories, Inc.; Hercules, CA, USA), while non-specific sites were blocked with either 5% non-fat dry milk or 5% bovine serum albumin (BSA) in Tris-buffered saline with Tween®-20 (TBST) buffer (150 mM NaCl, 100 mM Tris pH 7.5, and 0.1% (v/v) Tween-20) for 2 h at room temperature (RT). ..

    Article Title: Biological Evaluation of a Novel Compound with Predicted EZH2 and EED Binding Against Human Malignant Melanoma Cells
    Article Snippet: Next, whole cell extracts (60 μg) were prepared and subjected to electrophoresis on FastGene ® PAGE precast gels (gradient: 4–20%), using a FastGene ® PAGE Protein system (Nippon Genetics, Düren, Germany). .. Separated proteins were transferred to PVDF membranes using the semi-dry Trans-Blot ® TurboTM transfer system (Bio-Rad Laboratories, Inc.; Hercules, CA, USA), while non-specific sites were blocked with either 5% non-fat dry milk or 5% bovine serum albumin (BSA) in Tris-buffered saline with Tween ® -20 (TBST) buffer (150 mM NaCl, 100 mM Tris pH 7.5, and 0.1% ( v / v ) Tween-20) for 2 h at room temperature (RT). ..

    Article Title: Macrophages lacking TSC2 have mTORC1-dependent increased GPNMB and ameliorate ventricular dysfunction/remodeling after ischemia-reperfusion.
    Article Snippet: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used for protein separation using 4–15% Criterion TGX Gel 18 W 30ul (Bio-Rad Cat# 5671084) or 4–20% Criterion TGX Gel 26 W 15ul (Bio-Rad Cat# 5671095). .. Proteins were blotted onto nitrocellulose membrane 0.45 μm, 8.5 × 13.5 cm (Bio-Rad Cat1620167) using a semi-dry Trans-Blot® TurboTM Transfer System (BioRad Cat#1704150). .. Antibodies from Cell Signaling were used for P-P70S6 Kinase (9205, 1/000), P70S6 Kinase (9202, 1/1000), TSC2 (4308, 1/1000), P-4EBP1 (9451, 1/1000), 4EBP1 (9644, 1/1000), P-AKT (Ser473) (4060, 1/1000), P-AKT (Thr308) (5106, 1/1000), AKT (9272, 1/1000), and GPNMB (90205, 1/200).

    Article Title: Ion-Channel-Targeting Drugs for Chikungunya Virus
    Article Snippet: .. The lysate was then resolved using electrophoresis, followed by the transfer of the antigens to nitrocellulose membranes using a Semi-dry Trans-Blot ® TurboTM Transfer System (BIO-RAD; Hercules, CA, USA). .. The membranes were blocked with TBS-T (tris-buffered saline containing Tween-20) containing 1% low-fat milk, incubated overnight with a primary antibody (Anti-MBP, New England BioLabs), washed three times with TBS-T, incubated for two hours with a secondary antibody linked to horseradish peroxidase (anti-rabbit, Novagen; Temecula, CA, USA), and washed once again with TBS-T. Immunoreactive bands were detected using an ECL kit (Biological Industries; Haemek, Israel).

    Electrophoresis:

    Article Title: Ion-Channel-Targeting Drugs for Chikungunya Virus.
    Article Snippet: .. The lysate was then resolved using electrophoresis, followed by the transfer of the antigens to nitrocellulose membranes using a Semi-dry Trans-Blot® TurboTM Transfer System (BIO-RAD; Hercules, CA, USA). .. The membranes were blocked with TBS-T (tris-buffered saline containing Tween-20) containing 1% low-fat milk, incubated overnight with a primary antibody (Anti-MBP, New England BioLabs), washed three times with TBS-T, incubated for two hours with a secondary antibody linked to horseradish peroxidase (anti-rabbit, Novagen; Temecula, CA, USA), and washed once again with TBS-T. Immunoreactive bands were detected using an ECL kit (Biological Industries; Haemek, Israel).

    Article Title: Ion-Channel-Targeting Drugs for Chikungunya Virus
    Article Snippet: .. The lysate was then resolved using electrophoresis, followed by the transfer of the antigens to nitrocellulose membranes using a Semi-dry Trans-Blot ® TurboTM Transfer System (BIO-RAD; Hercules, CA, USA). .. The membranes were blocked with TBS-T (tris-buffered saline containing Tween-20) containing 1% low-fat milk, incubated overnight with a primary antibody (Anti-MBP, New England BioLabs), washed three times with TBS-T, incubated for two hours with a secondary antibody linked to horseradish peroxidase (anti-rabbit, Novagen; Temecula, CA, USA), and washed once again with TBS-T. Immunoreactive bands were detected using an ECL kit (Biological Industries; Haemek, Israel).

    Saline:

    Article Title: Biological Evaluation of a Novel Compound with Predicted EZH2 and EED Binding Against Human Malignant Melanoma Cells
    Article Snippet: Next, whole cell extracts (60 μg) were prepared and subjected to electrophoresis on FastGene® PAGE precast gels (gradient: 4–20%), using a FastGene® PAGE Protein system (Nippon Genetics, Düren, Germany). .. Separated proteins were transferred to PVDF membranes using the semi-dry Trans-Blot® TurboTM transfer system (Bio-Rad Laboratories, Inc.; Hercules, CA, USA), while non-specific sites were blocked with either 5% non-fat dry milk or 5% bovine serum albumin (BSA) in Tris-buffered saline with Tween®-20 (TBST) buffer (150 mM NaCl, 100 mM Tris pH 7.5, and 0.1% (v/v) Tween-20) for 2 h at room temperature (RT). ..

    Article Title: Biological Evaluation of a Novel Compound with Predicted EZH2 and EED Binding Against Human Malignant Melanoma Cells
    Article Snippet: Next, whole cell extracts (60 μg) were prepared and subjected to electrophoresis on FastGene ® PAGE precast gels (gradient: 4–20%), using a FastGene ® PAGE Protein system (Nippon Genetics, Düren, Germany). .. Separated proteins were transferred to PVDF membranes using the semi-dry Trans-Blot ® TurboTM transfer system (Bio-Rad Laboratories, Inc.; Hercules, CA, USA), while non-specific sites were blocked with either 5% non-fat dry milk or 5% bovine serum albumin (BSA) in Tris-buffered saline with Tween ® -20 (TBST) buffer (150 mM NaCl, 100 mM Tris pH 7.5, and 0.1% ( v / v ) Tween-20) for 2 h at room temperature (RT). ..



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